02603nas a2200253   4500008004100000245010100041210006900142260006600211300000800277490000800285520170100293653001301994653001802007653002202025653001902047653002102066100002402087700002402111700002202135700002402157700002102181700002202202856012502224       2006                        eng d00aDevelopment and evaluation of a DNA-barcoding approach for the rapid identification of nematodes0 aDevelopment and evaluation of a DNAbarcoding approach for the ra  aNORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANYbINTER-RESEARCH  a1-90 v3203 aFree-living nematodes are abundant in all marine habitats, are highly diverse, and can be useful for monitoring anthropogenic impacts on the environment. Despite such attributes, nematodes are effectively ignored by many marine ecologists because of their time-consuming taxonomy. Nematode diagnostics has traditionally relied on detailed comparison of morphological characters which, given their abundance, is difficult and laborious, meaning that the biodiversity of the group is typically underestimated. Molecular methods such as DNA-barcoding offer potentially efficient alternative approaches to studying the biodiversity of marine nematode communities, allowing these organisms to be more effectively exploited in ecological surveys and environmental assessments. In this study, a number of nuclear and mitochondrial genomic regions were evaluated as potential diagnostic loci for marine nematode species identification. Of these, the 18S ribosomal RNA gene amplified most reliably from a range of taxa, and was therefore evaluated as a DNA barcode. In a comparison of molecular and morphological identifications, over 97 % of specimens sequenced were correctly assigned on the basis of a short stretch of 18S rRNA sequence (approximately 345 bp), making this a potentially useful marker for the rapid molecular assignment of unknown nematode species, and evaluation of nematode species richness during ecological surveys or environmental assessments. This study showed that a single marker approach based on amplification and sequencing may prove invaluable in the rapid identification of nematodes during ecological surveys and, indeed, other taxonomically challenging invertebrate taxa.10a18S rRNA10aDNA barcoding10aecological survey10aidentification10aMarine nematodes1 aBhadury, Punyasloke1 aAusten, Melanie, C.1 aBilton, David, T.1 aLambshead, John, D.1 aRogers, Alex, D.1 aSmerdon, Gary, R.  uhttps://nematoda.myspecies.info/content/development-and-evaluation-dna-barcoding-approach-rapid-identification-nematodes01481nas a2200217   4500008004100000245010300041210006900144260007600213300001200289490000600301520067600307653001300983653002300996653001301019653001801032653001101050100002301061700002201084700002401106856013301130       2006                        eng d00aDevelopment of taxon-specific markers for high-throughput screening of microbial-feeding nematodes0 aDevelopment of taxonspecific markers for highthroughput screenin  a9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLANDbBLACKWELL PUBLISHING  a712-7140 v63 aIn an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets.10a18S rRNA10a5 ' nuclease assay10aNematoda10areal-time PCR10aTaqman1 aJones, Kenneth, L.1 aTodd, Timothy, C.1 aHerman, Michael, A.  uhttps://nematoda.myspecies.info/content/development-taxon-specific-markers-high-throughput-screening-microbial-feeding-nematodes