Direct colony identification by PCR-Miniprep

Publication Type:Journal Article
Year of Publication:2000
Authors:T. H. Dafa'alla, Hobom, G., Zahner, H.
Journal:Molecular Biology Today
Volume:1
Pagination:65-66
Abstract:

We have developed this PCR-based technique to directly identify transformed bacterial colonies before performing any DNA miniprep. Both specific as well as standard vector's primers can be used in this technique to analyze a large number of colonies in a relatively short time.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith