00896nas a2200193   4500008004100000245019700041210006900238260009000307300001200397490000700409100002300416700002600439700002400465700002100489700001700510700001700527700002100544856013700565       2008                        eng d00aSmall subunit ribosomal DNA-based phylogeny of basal Chromadoria (Nematoda) suggests that transitions from marine to terrestrial habitats (and vice versa) require relatively simple adaptations0 aSmall subunit ribosomal DNAbased phylogeny of basal Chromadoria   a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCE  a758-7630 v481 aHolterman, Martijn1 aHolovachov, Oleksandr1 avan den Elsen, Sven1 avan Megen, Hanny1 aBongers, Tom1 aBakker, Jaap1 aHelder, Johannes  uhttps://nematoda.myspecies.info/content/small-subunit-ribosomal-dna-based-phylogeny-basal-chromadoria-nematoda-suggests-transitions-03040nas a2200289   4500008004100000245013500041210006900176260007600245300001000321490000600331520198800337653002202325653001302347653001102360653002802371653002202399100002302421700002502444700002402469700002102493700001802514700002602532700001702558700001702575700002102592856013702613       2008                        eng d00aA ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats0 aribosomal DNAbased framework for the detection and quantificatio  a9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLANDbBLACKWELL PUBLISHING  a23-340 v83 aIndigenous communities of soil-resident nematodes have a high potential for soil health assessment as nematodes are diverse, abundant, trophically heterogeneous and easily extractable from soil. The conserved morphology of nematodes is the main operational reason for their under-exploitation as soil health indicators, and a user-friendly biosensor system should preferably be based on nonmorphological traits. More than 80% of the most environmental stress-sensitive nematode families belong to the orders Mononchida and Dorylaimida. The phylogenetic resolution offered by full-length small subunit ribosomal DNA (SSU rDNA) sequences within these two orders is highly different. Notwithstanding several discrepancies between morphology and SSU rDNA-based systematics, Mononchida families (indicated here as M1-M5) are relatively well-supported and, consequently, family-specific DNA sequences signatures could be defined. Apart from Nygolaimidae and Longidoridae, the resolution among Dorylaimida families was poor. Therefore, a part of the more variable large subunit rDNA (approximate to 1000 bp from the 5'-end) was sequenced for 72 Dorylaimida species. Sequence analysis revealed a subclade division among Dorylaimida (here defined as D1-D9, PP1-PP3) that shows only distant similarity with 'classical' Dorylaimid systematics. Most subclades were trophically homogeneous, and - in most cases - specific morphological characteristics could be pinpointed that support the proposed division. To illustrate the practicability of the proposed molecular framework, we designed primers for the detection of individual subclades within the order Mononchida in a complex DNA background (viz. in terrestrial or freshwater nematode communities) and tested them in quantitative assays (real-time polymerase chain reaction). Our results constitute proof-of-principle for the concept of DNA sequence signatures-based monitoring of stress sensitive nematode families in environmental samples.10aLSU ribosomal DNA10anematode10aRT-PCR10asoil community analysis10aSSU ribosomal DNA1 aHolterman, Martijn1 aRybarczyk, Katarzyna1 avan den Elsen, Sven1 avan Megen, Hanny1 aMooyman, Paul1 aSantiago, Reyes, Pena1 aBongers, Tom1 aBakker, Jaap1 aHelder, Johannes  uhttps://nematoda.myspecies.info/content/ribosomal-dna-based-framework-detection-and-quantification-stress-sensitive-nematode-familie