02121nas a2200217   4500008004100000245011200041210006900153260009000222300001200312490000700324520130300331653000801634653001501642653001201657653001001669653001501679653002501694100002001719700002701739856013701766       2007                        eng d00aPatterns of evolution of mitochondrial cytochrome c oxidase I and II DNA and implications for DNA barcoding0 aPatterns of evolution of mitochondrial cytochrome c oxidase I an  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCE  a325-3450 v443 aDNA barcoding has focused increasing attention on the use of specific regions of mitochondrial cytochrome c oxidase I and 11 genes (COI-COII) to diagnose and delimit species. However, our understanding of patterns of molecular evolution within these genes is limited. Here we examine patterns of nucleotide divergence in COI-COII within species and between species pairs of Lepidoptera and Diptera using a sliding window analysis. We found that: (1) locations of maximum divergence within COI-COII were highly variable among taxa surveyed in this study; (2) there was major overlap in divergence within versus between species, including within individual COI-CoII profiles; (3) graphical DNA saturation analysis showed variation in percent nucleotide transitions throughout COI-COII and only limited association with levels of DNA divergence. Ultimately, no single optimally informative 600 bp location was found within the 2.3kb of COI-COII, and the DNA barcoding region was no better than other regions downstream in Col. Consequently, we recommend that researchers should maximize sequence length to increase the probability of sampling regions of high phylogenetic informativeness, and to minimize stochastic variation in estimating total divergence. (c) 2007 Elsevier Inc. All rights reserved.10aCOI10adivergence10ainsects10amtDNA10asaturation10aspecies delimitation1 aRoe, Amanda, D.1 aSperling, Felix, A. H.  uhttps://nematoda.myspecies.info/content/patterns-evolution-mitochondrial-cytochrome-c-oxidase-i-and-ii-dna-and-implications-dna-barc02492nas a2200289   4500008004100000245014900041210006900190260007600259300000900335490000800344520150100352653000801853653000901861653001701870653001801887653001301905653000801918653002301926653001901949100001601968700001601984700001902000700002002019700001302039700001302052856013702065       2008                        eng d00aDisentangling taxonomy within the Rhabditis (Pellioditis) marina (Nematoda, Rhabditidae) species complex using molecular and morphological tools0 aDisentangling taxonomy within the Rhabditis Pellioditis marina N  a9600 GARSINGTON RD, OXFORD OX4 2ZG, OXON, ENGLANDbBLACKWELL PUBLISHING  a1-150 v1523 aCorrect taxonomy is a prerequisite for biological research, but currently it is undergoing a serious crisis, resulting in the neglect of many highly diverse groups of organisms. In nematodes, species delimitation remains problematic due to their high morphological plasticity. Evolutionary approaches using DNA sequences can potentially overcome the problems caused by morphology, but they are also affected by theoretical flaws. A holistic approach with a combination of morphological and molecular methods can therefore produce a straightforward delimitation of species. The present study investigates the taxonomic status of some highly divergent mitochondrial haplotypes in the Rhabditis (Pellioditis) marina species complex by using a combination of molecular and morphological tools. We used three molecular markers (COI, ITS, D2D3) and performed phylogenetic analyses. Subsequently, morphometric data from nearly all lineages were analysed with multivariate techniques. We included R. (P.) mediterranea and R. (R.) nidrosiensis to infer species status of the observed lineages. The results showed that highly divergent genotypic clusters were accompanied by morphological differences, and we created a graphical polytomous key for identifications. This study indisputably demonstrates that R. (P.) marina and R. (P.) mediterranea belong to a huge species complex and that biodiversity in free-living marine nematodes may be seriously underestimated. (C) 2008 The Linnean Society of London.10aCOI10aD2D310adelimitation10aDNA-barcoding10aholistic10aITS10aPellioditis marina10apolytomous key1 aDerycke, S.1 aFonseca, G.1 aVierstraete, A1 aVanfleteren, J.1 aVincx, M1 aMoens, T  uhttps://nematoda.myspecies.info/content/disentangling-taxonomy-within-rhabditis-pellioditis-marina-nematoda-rhabditidae-species-comp