Publication Type: | Journal Article |
Year of Publication: | 2006 |
Authors: | K. L. Jones, Todd, T. C., Herman, M. A. |
Journal: | Molecular Ecology Notes |
Volume: | 6 |
Pagination: | 712-714 |
Keywords: | 18S rRNA, 5 ' nuclease assay, Nematoda, real-time PCR, Taqman |
Abstract: | In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets. |
DOI: | 10.1111/j.1471-8286.2006.01319.x |
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